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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Mouse/Human/Rat Phospho-FAK / Focal Adhesion Kinase (Tyr397) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1506 Best Seller
Catalog
Size
Price
LS-F1506-1
1 plate
$590

Product Description

LS-F1506 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human FAK / Focal Adhesion Kinase in samples of Adherent Cell Cultures. It is based upon a Cell-Based Phosphorylation assay principle.

Specifications

Type
Cell-Based Phoshorylation ELISA (enzyme-linked immunosorbent assay) kit
Target
FAK / Focal Adhesion Kinase
Synonyms
PTK2, FADK, Fadk1, Focal adhesion kinase, FRNK, FADK 1, Focal adhesion kinase 1, FAK1, p125FAK, PPP1R71, Protein-tyrosine kinase 2, PTK2 protein tyrosine kinase 2, FAK, pp125 FA kinase, Pp125FAK
Reactivity
Mouse, Human, Rat
Specificity
FAK (Phospho-Tyr397)
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Qualitative
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • 96-Well Cell Culture Clear-Bottom Microplate
  • 10x TBS
  • Quenching Buffer
  • Blocking Buffer
  • 15x Wash Buffer
  • 100x Anti-FAK (Phospho-Tyr397) Antibody (Rabbit Polyclonal)
  • 100x Anti-FAK Antibody (Rabbit Polyclonal)
  • 100x Anti-GAPDH Antibody (Mouse Monoclonal)
  • HRP-Conjugated Anti-Rabbit IgG Antibody
  • HRP-Conjugated Anti-Mouse IgG Antibody
  • Primary Antibody Diluent
  • Ready-to-Use Substrate
  • Stop Solution
  • Crystal Violet Solution
  • SDS Solution
  • Adhesive Plate Seals
Protocol
                Restrictions
                For research use only.
                About FAK / Focal Adhesion Kinase
                Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Q05397 NM_005607 NP_005598.3


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                Cell-Based Phosphorylation ELISA Platform Overview
                Cell-Based Phosphorylation ELISA Platform Overview

                Cell-Based Phosphorylation ELISA Platform Overview
                Cell-Based Phosphorylation ELISA Platform Overview

                Cell-Based Phosphorylation ELISA Platform Overview
                Cell-Based Phosphorylation ELISA Platform Overview

                Cell-Based Phosphorylation ELISA Platform Overview
                Cell-Based Phosphorylation ELISA Platform Overview

                Requested From: United States
                Date Requested: 10/22/2018

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