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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Mouse/Human/Rat Phospho-CRYAB / Alpha B Crystallin (Ser45) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1349
LS-F1349 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human CRYAB / Alpha B Crystallin in samples of Adherent Cell Cultures. It is based upon a Cell-Based Phosphorylation assay principle.
Catalog
Size
Price
LS-F1349-1
1 plate
$590

Popular CRYAB / Alpha B Crystallin Elisa Kits

Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
Reactivity: Human
Range: 0.16-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma
Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
Reactivity: Mouse
Range: 0.157-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Mouse
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Lysates, Tissue Homogenates
Type: Cell-Based Phosphorylation ELISA
Format: 96-Well Microplate
Reactivity: Mouse, Human, Rat
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures

Product Description

LS-F1349 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human CRYAB / Alpha B Crystallin in samples of Adherent Cell Cultures. It is based upon a Cell-Based Phosphorylation assay principle.
About CRYAB / Alpha B Crystallin
Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. P02511 NM_001885 NP_001876.1

CRYAB ElisaKit, Alpha-crystallin B chain ElisaKit, CRYA2 ElisaKit, Crystallin, alpha B ElisaKit, CTPP2 ElisaKit, Heat shock protein beta-5 ElisaKit, HSPB5 ElisaKit, Alpha B Crystallin ElisaKit, Alpha(B)-crystallin ElisaKit, Rosenthal fiber component ElisaKit, Heat-shock 20 kD like-protein ElisaKit


Specifications

Type
Cell-Based Phoshorylation ELISA (enzyme-linked immunosorbent assay) kit
Target
CRYAB / Alpha B Crystallin
Reactivity
Mouse, Human, Rat
Specificity
CRYAB (Phospho-Ser45)
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • 96-Well Cell Culture Clear-Bottom Microplate
  • 10x TBS
  • Quenching Buffer
  • Blocking Buffer
  • 15x Wash Buffer
  • 100x Anti-CRYAB (Phospho-Ser45) Antibody (Rabbit Polyclonal)
  • 100x Anti-CRYAB Antibody (Rabbit Polyclonal)
  • 100x Anti-GAPDH Antibody (Mouse Monoclonal)
  • HRP-Conjugated Anti-Rabbit IgG Antibody
  • HRP-Conjugated Anti-Mouse IgG Antibody
  • Primary Antibody Diluent
  • Ready-to-Use Substrate
  • Stop Solution
  • Crystal Violet Solution
  • SDS Solution
  • Adhesive Plate Seals
Protocol
                Restrictions
                For research use only.
                Guarantee
                This elisa kit carries the LSBio 100% Guarantee.

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                Images

                Cell-Based Phosphorylation ELISA Platform Overview
                Cell-Based Phosphorylation ELISA Platform Overview

                Cell-Based Phosphorylation ELISA Platform Overview
                Cell-Based Phosphorylation ELISA Platform Overview

                Cell-Based Phosphorylation ELISA Platform Overview
                Cell-Based Phosphorylation ELISA Platform Overview

                Cell-Based Phosphorylation ELISA Platform Overview
                Cell-Based Phosphorylation ELISA Platform Overview

                Requested From: United States
                Date Requested: 8/20/2018

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