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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Mouse/Human/Rat NOS3 / eNOS ELISA Kit (Cell-Based ELISA) - LS-F3310
LS-F3310 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human NOS3 / eNOS in samples of Adherent Cell Cultures. It is based upon a Cell-Based assay principle.
Catalog
Size
Price
LS-F3310-1
1 plate
$490

Popular NOS3 / eNOS Elisa Kits

Type: Cell-Based Phosphorylation ELISA
Format: 96-Well Microplate
Reactivity: Mouse, Human, Rat
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Rat
Range: 1.56-100 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Lysates, Serum, Tissue Homogenates, Plasma
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Mouse
Range: 1.56-100 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Tissue Homogenates, Plasma
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 78-5000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma
Type: Cell-Based Phosphorylation ELISA
Format: 96-Well Microplate
Reactivity: Mouse, Human, Rat
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures

Product Description

LS-F3310 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human NOS3 / eNOS in samples of Adherent Cell Cultures. It is based upon a Cell-Based assay principle.
About NOS3 / eNOS
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. P29474 NM_000603 NP_000594.2

NOS3 ElisaKit, Constitutive NOS ElisaKit, CNOS ElisaKit, Endothelial NOS ElisaKit, ECNOS ElisaKit, NOS type III ElisaKit, NOSIII ElisaKit, EC-NOS ElisaKit, ENOS ElisaKit, Nitric oxide synthase 3 ElisaKit


Specifications

Type
Cell-Based ELISA (enzyme-linked immunosorbent assay) kit
Target
NOS3 / eNOS
Reactivity
Mouse, Human, Rat
Specificity
eNOS
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • 96-Well Cell Culture Clear-Bottom Microplate
  • 10x TBS
  • Quenching Buffer
  • Blocking Buffer
  • 10x Wash Buffer
  • 100x Anti-eNOS Antibody (Rabbit Polyclonal)
  • 100x Anti-GAPDH Antibody (Mouse Monoclonal)
  • HRP-Conjugated Anti-Rabbit IgG Antibody
  • HRP-Conjugated Anti-Mouse IgG Antibody
  • Primary Antibody Diluent
  • Ready-to-Use Substrate
  • Stop Solution
  • Crystal Violet Solution
  • SDS Solution
  • Adhesive Plate Seals
Protocol
                    Restrictions
                    For research use only.
                    Guarantee
                    This elisa kit carries the LSBio 100% Guarantee.

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                    Images

                    Cell-Based ELISA Platform Overview
                    Cell-Based ELISA Platform Overview

                    Cell-Based ELISA Platform Overview
                    Cell-Based ELISA Platform Overview

                    Cell-Based ELISA Platform Overview
                    Cell-Based ELISA Platform Overview

                    Cell-Based ELISA Platform Overview
                    Cell-Based ELISA Platform Overview

                    Requested From: United States
                    Date Requested: 8/21/2018

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