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Mouse/Human/Rat NEP / DDR1 ELISA Kit (Cell-Based ELISA) - LS-F1939

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1 plate

Product Description

LS-F1939 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human NEP / DDR1 in samples of Adherent Cell Cultures. It is based upon a Cell-Based assay principle.


Cell-Based ELISA (enzyme-linked immunosorbent assay) kit
Mouse, Human, Rat
Intended Sample Types
Adherent Cell Cultures
96-Well Microplate
Colorimetric - 450nm (TMB)
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • User Manual
  • 96-well Cell Culture Microplate
  • Blocking Buffer
  • Detection Antibodies
  • HRP-Conjugate
  • Substrate
  • Stop Solution
  • Crystal Violet Solution
  • SDS Solution
  • Adhesive Plate Sealers
  1. Seed cells into wells and incubate overnight at 37°C, 5% CO2.
  2. Apply desired treatment conditions.
  3. Add 100 µl of Fixing Solution and incubate 20 minutes at room temperature.
  4. Add 100 µl of Quenching Buffer and incubate 20 minutes at room temperature.
  5. Add 200 µl of Blocking Buffer and incubate for 1 hour at room temperature.
  6. Add 50 µl of 1x primary antibodies and incubate overnight at 4°C. Add 50 µl of HRP-conjugated secondary antibodies and incubate for 1.5 hours at room temperature.
  7. Add 50 µl of Ready-to-Use Substrate and incubate for 30 minutes at room temperature.
For research use only.
About NEP / DDR1
Q08345 NM_013994 NP_054700.2

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Cell-Based ELISA Platform Overview
Cell-Based ELISA Platform Overview

Cell-Based ELISA Platform Overview
Cell-Based ELISA Platform Overview

Cell-Based ELISA Platform Overview
Cell-Based ELISA Platform Overview

Cell-Based ELISA Platform Overview
Cell-Based ELISA Platform Overview

Requested From: United States
Date Requested: 7/16/2019
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