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Mouse/Human/Rat GHRL / Ghrelin Preproprotein ELISA Kit (Competitive EIA) - LS-F6
Catalog
Size
Price
LS-F6-1
1 plate
$440
Available Discounts
10% off 2-4 units
20% off 5+ units

Popular GHRL / Ghrelin Preproprotein Elisa Kits


Product Description

LS-F6 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human GHRL / Ghrelin Preproprotein in samples of Cell Culture Supernatants, Cell Lysates, Plasma and Serum. It is based upon a Competitive EIA assay principle and can be used to detect levels of GHRL / Ghrelin Preproprotein as low as 0.1 nanograms per millilter.

Specifications

Type
Competitive EIA (enzyme immunoassay) kit
Target
GHRL / Ghrelin Preproprotein
Synonyms
GHRL, Ghrelin, Growth hormone secretagogue, Motilin-related peptide, Obestatin, Appetite-regulating hormone, MTLRP, Protein M46
Reactivity
Mouse, Human, Rat
Specificity
Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Nesfatin, Angiotensin II, NPY and APC.
Manual
Intended Sample Types
Cell Culture Supernatants, Cell Lysates, Plasma, Serum
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.1 - 1000 ng/ml
Sensitivity
0.1 ng/ml
Precision
Intra-Assay: CV<10% Inter-Assay: CV<15%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Ghrelin Microplate - Item A
  • Wash Buffer Concentrate (20x) - Item B
  • Lyophilized standard Ghrelin peptide - Item C
  • Lyophilized anti-Ghrelin polyclonal antibody - Item N
  • Assay Diluent E (1x) - Item R
  • Lyophilized biotinylated Ghrelin peptide - Item F
  • HRP-Streptavidin concentrate - Item G
  • Lyophilized positive control - Item M
  • TMB Substrate
  • Stop Solution - Item I
  • Adhesive Plate Sealers
Protocol
              Restrictions
              For research use only.
              About GHRL / Ghrelin Preproprotein
              GHRL / Ghrelin Preproprotein is the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Q9UBU3 NM_016362 NP_057446.1


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              Competition ELISA Platform Overview
              Competition ELISA Platform Overview

              Competition ELISA Platform Overview
              Competition ELISA Platform Overview

              Competition ELISA Platform Overview
              Competition ELISA Platform Overview

              Competition ELISA Platform Overview
              Competition ELISA Platform Overview

              Requested From: United States
              Date Requested: 10/16/2018

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