Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
The capture antibody provided in this kit recognizes human, mouse and rat GHRL / Ghrelin.
LS-F11 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human GHRL / Ghrelin Preproprotein in samples of Cell Culture Supernatants, Cell Lysates, Plasma and Serum. It is based upon a Competitive EIA assay principle.
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Obestatin Microplate - Item A
Wash Buffer Concentrate (20x) - Item B
Standard Obestatin Peptide - Item C
Anti-Obestatin polyclonal antibody - Item N
Assay Diluent A - Item D
Assay Diluent B - Item E
Biotinylated Obestatin peptide, - Item F
HRP-Streptavidin concentrate - Item G
Positive control - Item M
Stop Solution - Item I
Adhesive Plate Sealers
Standard, Biotinylated Obestatin peptide, and Positive Control should be stored at -20°C or -80°C (recommended at -80°C) after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at -20°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge. If stored in this manner, Lifespan warranties this kit for 6 months from the date of shipment.
GHRL / Ghrelin Preproprotein is the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin).