Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
The capture antibody provided in this kit recognizes human, mouse and rat GIP.
LS-F7 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Gastric Inhibitory Peptide in samples of Cell Culture Supernatants, Cell Lysates, Plasma and Serum. It is based upon a Competitive EIA assay principle.
All LSBio Custom kits have been predesigned and all necessary components, including antibodies and standards have been identified. Upon receiving a custom kit order, the kit will be assembled and quality control tested before being shipped out. Kit assembly and testing typically takes 4 to 6 weeks. In most cases the final kit is based on the Sandwich assay principle, with a few being Competitive EIA based. Specifications such as Range, Sensitivity, and Precision are defined upon completion. In the event that the custom kit cannot be successfully developed with 6 weeks of the order date, the customer will be notified and the order canceled at no cost.
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
GIP Microplate - Item A
Wash Buffer Concentrate (20x) - Item B
Standard GIP Peptide - Item C
Anti-GIP polyclonal antibody - Item N
Assay Diluent A - Item D
Assay Diluent B - Item E
Biotinylated GIP peptide, - Item F
HRP-Streptavidin concentrate - Item G
Positive control - Item M
Stop Solution - Item I
Adhesive Plate Sealers
Standard, Biotinylated GIP peptide, and Positive Control should be stored at -20°C or -80°C (recommended at -80°C) after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at -20°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge. If stored in this manner, Lifespan warranties this kit for 6 months from the date of shipment.
Gastric Inhibitory Peptide is an incretin hormone and belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption. This gene stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion.