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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Mouse/Human/Rat CDK5R1 ELISA Kit (Cell-Based ELISA) - LS-F3199
Catalog
Size
Price
LS-F3199-1
1 plate
$490

Product Description

LS-F3199 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human CDK5R1 in samples of Adherent Cell Cultures. It is based upon a Cell-Based assay principle.

Specifications

Type
Cell-Based ELISA (enzyme-linked immunosorbent assay) kit
Target
CDK5R1
Synonyms
CDK5R1, CDK5R, CDK5P35, p35, p35nck5a, p23, p25, TPKII regulatory subunit, CDK5 activator 1, NCK5A, Neuronal CDK5 activator
Reactivity
Mouse, Human, Rat
Specificity
CDK5R1
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Qualitative
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • 96-Well Cell Culture Clear-Bottom Microplate
  • 10x TBS
  • Quenching Buffer
  • Blocking Buffer
  • 10x Wash Buffer
  • 100x Anti-CDK5R1 Antibody (Rabbit Polyclonal)
  • 100x Anti-GAPDH Antibody (Mouse Monoclonal)
  • HRP-Conjugated Anti-Rabbit IgG Antibody
  • HRP-Conjugated Anti-Mouse IgG Antibody
  • Primary Antibody Diluent
  • Ready-to-Use Substrate
  • Stop Solution
  • Crystal Violet Solution
  • SDS Solution
  • Adhesive Plate Seals
Protocol
                    Restrictions
                    For research use only.
                    About CDK5R1
                    CDK5R1 (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. Q15078 NM_003885 NP_003876.1


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                    Cell-Based ELISA Platform Overview
                    Cell-Based ELISA Platform Overview

                    Cell-Based ELISA Platform Overview
                    Cell-Based ELISA Platform Overview

                    Cell-Based ELISA Platform Overview
                    Cell-Based ELISA Platform Overview

                    Cell-Based ELISA Platform Overview
                    Cell-Based ELISA Platform Overview

                    Requested From: United States
                    Date Requested: 10/22/2018

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