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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Mouse/Human/Rat BAX ELISA Kit (Cell-Based ELISA) - LS-F1820
Catalog
Size
Price
LS-F1820-1
1 plate
$490

Popular BAX Elisa Kits

Reactivity: Rat
Range: 0.312-20 ng/ml
Reactivity: Mouse
Range: 31.25-2000 pg/ml
Reactivity: Rat
Range: 12.5-800 pg/ml
Reactivity: Mouse
Range: 62.5-4000 pg/ml

Product Description

LS-F1820 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human BAX in samples of Adherent Cell Cultures. It is based upon a Cell-Based assay principle.

Specifications

Type
Cell-Based ELISA (enzyme-linked immunosorbent assay) kit
Target
BAX
Synonyms
BAX, BCL2-associated X protein, Bcl2-L-4, Apoptosis regulator BAX, Bcl-2-like protein 4, BCL2L4
Reactivity
Mouse, Human, Rat
Specificity
BAX
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Qualitative
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • 96-Well Cell Culture Clear-Bottom Microplate
  • 10x TBS
  • Quenching Buffer
  • Blocking Buffer
  • 10x Wash Buffer
  • 100x Anti-BAX Antibody (Rabbit Polyclonal)
  • 100x Anti-GAPDH Antibody (Mouse Monoclonal)
  • HRP-Conjugated Anti-Rabbit IgG Antibody
  • HRP-Conjugated Anti-Mouse IgG Antibody
  • Primary Antibody Diluent
  • Ready-to-Use Substrate
  • Stop Solution
  • Crystal Violet Solution
  • SDS Solution
  • Adhesive Plate Seals
Protocol
                Restrictions
                For research use only.
                About BAX
                Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Q07812 NM_004324 NP_004315.1


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                Cell-Based ELISA Platform Overview
                Cell-Based ELISA Platform Overview

                Cell-Based ELISA Platform Overview
                Cell-Based ELISA Platform Overview

                Cell-Based ELISA Platform Overview
                Cell-Based ELISA Platform Overview

                Cell-Based ELISA Platform Overview
                Cell-Based ELISA Platform Overview

                Requested From: United States
                Date Requested: 10/17/2018

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