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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Mouse/Human/Rat ALOX5 / 5-LOX ELISA Kit (Cell-Based ELISA) - LS-F1791
LS-F1791 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human ALOX5 / 5-LOX in samples of Adherent Cell Cultures. It is based upon a Cell-Based assay principle.
Catalog
Size
Price
LS-F1791-1
1 plate
$490

Popular ALOX5 / 5-LOX Elisa Kits

Type: Cell-Based Phosphorylation ELISA
Format: 96-Well Microplate
Reactivity: Mouse, Human, Rat
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Lysates, Tissue Homogenates
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Rat
Range: 0.781-50 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Lysates, Tissue Homogenates
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Mouse
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

Product Description

LS-F1791 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human ALOX5 / 5-LOX in samples of Adherent Cell Cultures. It is based upon a Cell-Based assay principle.
About ALOX5 / 5-LOX
ALOX5 / 5-LOX is a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). P09917 NM_000698 NP_000689.1

ALOX5 ElisaKit, 5-lipoxygenase ElisaKit, 5LPG ElisaKit, 5-LO ElisaKit, 5-LOX ElisaKit, Arachidonate 5-lipoxygenase ElisaKit, LOG5 ElisaKit, LOX-5 ElisaKit, Leukotriene A4 synthase ElisaKit


Specifications

Type
Cell-Based ELISA (enzyme-linked immunosorbent assay) kit
Target
ALOX5 / 5-LOX
Reactivity
Mouse, Human, Rat
Specificity
ALOX5
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • 96-Well Cell Culture Clear-Bottom Microplate
  • 10x TBS
  • Quenching Buffer
  • Blocking Buffer
  • 10x Wash Buffer
  • 100x Anti-ALOX5 Antibody (Rabbit Polyclonal)
  • 100x Anti-GAPDH Antibody (Mouse Monoclonal)
  • HRP-Conjugated Anti-Rabbit IgG Antibody
  • HRP-Conjugated Anti-Mouse IgG Antibody
  • Primary Antibody Diluent
  • Ready-to-Use Substrate
  • Stop Solution
  • Crystal Violet Solution
  • SDS Solution
  • Adhesive Plate Seals
Protocol
                Restrictions
                For research use only.
                Guarantee
                This elisa kit carries the LSBio 100% Guarantee.

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                Images

                Cell-Based ELISA Platform Overview
                Cell-Based ELISA Platform Overview

                Cell-Based ELISA Platform Overview
                Cell-Based ELISA Platform Overview

                Cell-Based ELISA Platform Overview
                Cell-Based ELISA Platform Overview

                Cell-Based ELISA Platform Overview
                Cell-Based ELISA Platform Overview

                Requested From: United States
                Date Requested: 8/15/2018

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