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Mouse Histocompatibility 2, Class II antigen A, alpha (H2-Aa) ELISA Kit (Competitive EIA) - LS-F40022

Catalog Size Price
LS-F40022-1 1 plate Unavailable

Most Popular Mouse Histocompatibility 2, Class II antigen A, alpha (H2-Aa) Elisa Kits

Mouse Histocompatibility 2, Class II antigen A, alpha (H2-Aa) ELISA Kit (Competitive EIA) - LS-F40022
Quantitative Competitive EIA
96-Well Strip Plate
Mouse
0.625-40 ng/ml
Colorimetric - 450nm (TMB)
Serum, Tissue Homogenates, Plasma

100% Guaranteed
LS-F40022
Competitive EIA (enzyme immunoassay) kit
Mouse Histocompatibility 2, Class II antigen A, alpha (H2-Aa)
Mouse
LS-F40022 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse Mouse Histocompatibility 2, Class II antigen A, alpha (H2-Aa) in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Competitive EIA assay principle and can be used to detect levels of Mouse Histocompatibility 2, Class II antigen A, alpha (H2-Aa) as low as 0.375 nanograms per millilter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
0.625 - 40 ng/ml
0.375
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Coated 96-well Strip Plate
  • Adhesive Plate Sealers
  • Sample Diluent
  • Wash Buffer (25x)
  • TMB Substrate
  • Stop Solution
  • Standard (Lyophilized)
  • HRP Conjugate Diluent
  • Biotinylated Detection Antibody Diluent
  • HRP Conjugate (100x)
  • Biotinylated Detection Antibody (100x)
    This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with H2-Aa. During the reaction, H2-Aa in the sample or standard competes with a fixed amount of H2-Aa on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to H2-Aa. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of H2-Aa in the samples is then determined by comparing the O.D. of the samples to the standard curve.
For research use only.

About Mouse Histocompatibility 2, Class II antigen A, alpha (H2-Aa)


 

Competition ELISA Platform Overview
Competition ELISA Platform Overview

Requested From: 
Date Requested: 5/25/2018

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