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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Mouse F9 / Factor IX ELISA Kit (Sandwich ELISA) - LS-F36732
Catalog
Size
Price
LS-F36732-1
1 plate
$790

Popular F9 / Factor IX Elisa Kits

Reactivity: Mouse
Range: 0.16-10 ng/ml
Reactivity: Mouse
Range: 1.56-100 ng/ml
Reactivity: Rat
Range: 0.5-10 ng/ml
Reactivity: Monkey
Range: 0.313-20 ng/ml
Reactivity: Human
Range: 1.25-80 ng/ml

Product Description

LS-F36732 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse F9 / Factor IX in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of F9 / Factor IX as low as 0.094 nanograms per millilter.

Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
F9 / Factor IX
Synonyms
F9, Christmas Disease, Coagulation factor IX, Factor IX Deficiency, FIX, HEMB, F9 p22, Factor 9, Factor IX, Factor IX F9, FIX F9, Haemophilia B, THPH8, Christmas factor, Hemophilia B, PTC, Serine protease
Reactivity
Mouse
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.156 - 10 ng/ml
Sensitivity
0.094 ng/ml
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
Store at 4°C. Stable for 6 months.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
Background
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- FIX antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-FIX antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FIX amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofFIX can be calculated.
Restrictions
For research use only.
About F9 / Factor IX
F9 / Factor IX is a vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. P00740 NM_000133 NP_000124.1


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Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 10/17/2018

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