Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Recognizes mouse prothrombin, thrombin, and thrombin/antithrombin complex. Significant cross reaction is observed with pooled normal plasma from rat. Pooled normal plasma from human, porcine, rabbit, and sheep were assayed for cross-reactivity. No significant cross-reactivity was observed.
LS-F10448 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse F2 / Prothrombin / Thrombin in samples of Cell Culture Media, Plasma, Serum and Tissue Extracts. It is based upon a Sandwich assay principle and can be used to detect levels of F2 / Prothrombin / Thrombin as low as 0.319 nanograms per millilter.
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Prothrombin (aka Factor II) is a single-chain vitamin K dependent 579 amino acid glycoprotein zymogen. Prothrombin is proteolytically activated to thrombin by the prothrombinase enzyme complex in the coagulation cascade common pathway. The serine protease thrombin converts plasma fibrinogen to insoluble fibrin. Prothrombin levels are decreased by anticoagulant therapy, vitamin K deficiency and severe liver disease. Elevated plasma prothrombin is associated with a single nucleotide change at position 20210.
Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Finally, peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia.