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Mouse F2 / Prothrombin / Thrombin ELISA Kit (Sandwich ELISA) - LS-F10448

Catalog Size Price
LS-F10448-1 1 plate Unavailable

Most Popular F2 / Prothrombin / Thrombin Elisa Kits

Human F2 / Prothrombin / Thrombin ELISA Kit (Sandwich ELISA) - LS-F4268
Sandwich ELISA
96-Well Strip Plate
Human
4.69-300 ng/ml
Colorimetric - 450nm (TMB)
Serum
 Image
Human F2 / Prothrombin / Thrombin ELISA Kit (Sandwich ELISA) - LS-F13466
Quantitative Sandwich ELISA
96-Well Strip Plate
Human
0.78-50 ng/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Serum, Tissue Homogenates, Plasma
 Image
Rat F2 / Prothrombin / Thrombin ELISA Kit (Sandwich ELISA) - LS-F13467
Quantitative Sandwich ELISA
96-Well Strip Plate
Rat
1.56-100 ng/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Serum, Tissue Homogenates, Plasma
 Image
Bovine F2 / Prothrombin / Thrombin ELISA Kit (Sandwich ELISA) - LS-F13468
Quantitative Sandwich ELISA
96-Well Strip Plate
Bovine
7.8-500 ng/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Serum, Tissue Homogenates, Plasma
 Image
Human F2 / Prothrombin / Thrombin ELISA Kit (Sandwich ELISA) - LS-F22981
Quantitative Sandwich ELISA
96-Well Microplate
Human
15.63-1000 pg/ml
Colorimetric - 450nm (TMB)
Serum, Plasma
 Image

100% Guaranteed
LS-F10448
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
F2 / Prothrombin / Thrombin
Mouse
Recognizes mouse prothrombin, thrombin, and thrombin/antithrombin complex. Significant cross reaction is observed with pooled normal plasma from rat. Pooled normal plasma from human, porcine, rabbit, and sheep were assayed for cross-reactivity. No significant cross-reactivity was observed.
LS-F10448 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse F2 / Prothrombin / Thrombin in samples of Cell Culture Media, Plasma, Serum and Tissue Extracts. It is based upon a Sandwich assay principle and can be used to detect levels of F2 / Prothrombin / Thrombin as low as 0.319 nanograms per millilter.
Cell Culture Media, Plasma, Serum, Tissue Extracts
1:10,000-1:40,000 (Plasma)
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
1 - 500 ng/ml
0.319 ng/ml
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • 96-well antibody coated microtiter strip plate
  • 10X Wash buffer
  • 5X Diluent
  • Mouse prothrombin standard (Lyophilized)
  • Anti-mouse prothrombin / thrombin primary antibody
  • Horseradish peroxidase-conjugated streptavidin
  • TMB Substrate
  • Adhesive Plate Sealers
    Prothrombin (aka Factor II) is a single-chain vitamin K dependent 579 amino acid glycoprotein zymogen. Prothrombin is proteolytically activated to thrombin by the prothrombinase enzyme complex in the coagulation cascade common pathway. The serine protease thrombin converts plasma fibrinogen to insoluble fibrin. Prothrombin levels are decreased by anticoagulant therapy, vitamin K deficiency and severe liver disease. Elevated plasma prothrombin is associated with a single nucleotide change at position 20210.
Store at 4°C.
For research use only.

About F2 / Prothrombin / Thrombin

P00734 NM_000506 NP_000497.1

F2 ElisaKit, Prothrombin B-chain ElisaKit, Coagulation factor II ElisaKit, FACTOR II ElisaKit, Prothrombin ElisaKit, PT ElisaKit, RPRGL2 ElisaKit, Serine protease ElisaKit, THPH1 ElisaKit, Thrombin ElisaKit

Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Finally, peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia.

 

Sandwich ELISA Platform Overview

Requested From: 
Date Requested: 10/21/2017

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