Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
The antibody pair provided in this kit recognizes mouse Complement Component C5a.
LS-F537 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse Complement C5a in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of Complement C5a as low as 1.64 picograms per milliliter.
Cell Culture Supernatants, Plasma, Serum
Colorimetric - 450nm (TMB)
1.64 - 400 pg/ml
Intra-Assay: CV<10% Inter-Assay: CV<12%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Coated 96-well Strip Plate
Wash Buffer Concentrate (20X)
Biotinylated Detection Antibody
HRP-Streptavidin Conjugate (200x)
Assay Diluent A
Assay Diluent B (5x)
Adhesive Plate Sealers
May be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standard should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze/thaw cycles.
For research use only.
About Complement C5a
Complement component 5 is a protein that in humans is encoded by the C5 gene. Complement component 5 is involved in the complement system. It is cleaved into C5a and C5b. C5a plays an important role in chemotaxis. C5b forms the first part of the complement membrane attack complex. Deficiency is thought to cause Leiner's disease.