Sandwich ELISA (enzyme-linked immunosorbent assay) kit
This kit is for the detection of natural and recombinant mouse Complement C5a. No significant cross-reactivity or interference between Complement C5a and analogs was observed.
Intended Sample Types
Cell Culture Supernatants, Plasma, Serum
Colorimetric - 450nm (TMB)
15.6 - 1000 pg/ml
Intra-Assay: CV<5.3% Inter-Assay: CV<7.7%
Short term: 4°C; Long term: see manual.
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
- Coated 96-well Strip Plate
- Standard (Lyophilized)
- Biotinylated Detection Antibody (100x)
- ABC Complex (100x)
- Sample Diluent
- Detection Antibody Diluent
- ABC Complex Diluent
- TMB Substrate
- Stop Solution
- Adhesive Plate Sealers
For research use only.
About Complement C5a
C5a is a protein fragment released from cleavage complement component C5 by protease C5-convertase into C5a and C5b fragments. C5b is important in late events of complement cascade, whereas C5a acts as highly inflammatory peptide. The origin of C5 is in the hepatocyte but its synthesis can also be found in macrophages that may cause local increase of C5a. C5a has chemotactic and anaphylatoxic properties, it is essential in the innate immunity but it is also linked with the adaptive immunity.