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Mouse CCL17 / TARC ELISA Kit (Sandwich ELISA) - LS-F10150

Catalog Size Price
LS-F10150-1 1 plate Unavailable
Available Discounts 10% off 5+ units

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100% Guaranteed
LS-F10150
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
CCL17 / TARC
Mouse
This assay has high sensitivity and excellent specificity for detection of mouse TARC. No significant cross-reactivity or interference between mouse TARC and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between mouse TARC and all the analogs, therefore, cross reaction may still exist.
LS-F10150 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse CCL17 / TARC in samples of Cell Culture Supernatants, Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CCL17 / TARC as low as 10.512 picograms per milliliter.
Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates
50 - 100 µl
96-Well Microplate
Quantitative
31.25 - 2000 pg/ml
10.512 pg/ml
Intra-assay: CV%<8% Inter-assay: CV%<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Assay plate (12 x 8 coated Microwells)
  • Standard (Lyophilized)
  • Biotin-antibody (100 x concentrate)
  • HRP-avidin (100 x concentrate)
  • Biotin-antibody Diluent
  • HRP-avidin Diluent
  • Sample Diluent
  • Wash Buffer (25 x concentrate)
  • TMB Substrate
  • Stop Solution
  • Adhesive Strip (For 96 wells)
    This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TARC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TARC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TARC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TARC bound in the initial step. The color development is stopped and the intensity of the color is measured.
2°C to 8°C
For research use only.

About CCL17 / TARC

Q92583 NM_002987 NP_002978.1

CCL17 ElisaKit, A-152E5.3 ElisaKit, C-C motif chemokine 17 ElisaKit, CC chemokine abcd-2 ElisaKit, ABCD-2 ElisaKit, SCYA17 ElisaKit, T cell-directed CC chemokine ElisaKit, TARC ElisaKit, CC chemokine TARC ElisaKit, Small-inducible cytokine A17 ElisaKit

This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for T lymphocytes, but not monocytes or granulocytes. The product of this gene binds to chemokine receptors CCR4 and CCR8.

 

Sandwich ELISA Platform Overview

Requested From: 
Date Requested: 10/20/2017

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