Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
This assay has high sensitivity and excellent specificity for detection of human VB12. No significant cross-reactivity or interference between human VB12 and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human VB12 and all the analogs, therefore, cross reaction may still exist.
LS-F10055 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Vitamin B12 / Cyanocobalamin in samples of Plasma and Serum. It is based upon a Competitive EIA assay principle and can be used to detect levels of Vitamin B12 / Cyanocobalamin as low as 1.498 picograms per milliliter.
50 - 100 µl
1.56 - 100 pg/ml
Intra-assay: CV%<8% Inter-assay: CV%<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
2 x Standard
Biotin-conjugate (10 x concentrate)
HRP-avidin (100 x concentrate)
2 x Sample Diluent
Wash Buffer (25 x concentrate)
Adhesive Strip (For 96 wells)
This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for VB12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated VB12. A competitive inhibition reaction is launched between VB12 (Standards or samples) and biotin-conjugated VB12 with the pre-coated VB12 antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of VB12 bound in the initial step. The color development is stopped and the intensity of the color is measured.