Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
"The Human TNF-alpha ELISA is capable of recognizing both recombinant and naturally produced Human TNF-alpha proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 1% cross reactivity.• Rat: TNF-alphaThe antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human: 4-1BBL, 4-1BBR, BAFF, CD40L, sFasL, sRANKL, sTNF-RI, sTNF-RII, TNF-beta, sTRAIL, sTRAIL-RI, TWEAK, TWEAK-R, VEGF"
LS-F600 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human TNF Alpha in samples of Cell Lysates, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of TNF Alpha as low as 16 picograms per milliliter.
Cell Lysates, Plasma, Serum
12 x 8-Well Microstrips
Colorimetric - 450nm (TMB)
16 - 2000 pg/ml
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
TNF Alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.