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Human TNF Alpha ELISA Kit (Sandwich ELISA) - LS-F24688


Size Price
1 plate $550
Inquire for larger quantities
LSBio (Direct) LSBio (Direct)

Most Popular TNF Alpha Elisa Kits

Rabbit TNF Alpha ELISA Kit (Sandwich ELISA) - LS-F5127
Sandwich ELISA
96-Well Strip Plate
3.13-200 pg/ml
Colorimetric - 450nm
Cell Culture Supernatants, Cell Lysates, Serum, Tissue Homogenates, Plasma
Guinea pig TNF Alpha ELISA Kit (Sandwich ELISA) - LS-F5322
Sandwich ELISA
96-Well Strip Plate
Guinea pig
7.8-500 pg/ml
Colorimetric - 450nm
Cell Culture Supernatants, Cell Lysates, Serum, Tissue Homogenates, Plasma
Mouse TNF Alpha ELISA Kit (Sandwich ELISA) - LS-F12798
Quantitative Sandwich ELISA
96-Well Strip Plate
7.8-500 pg/ml
Cell Culture Supernatants, Serum, Tissue Homogenates, Plasma

100% Guaranteed 100% Guaranteed
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
TNF Alpha
For the detection and quantification of endogenous levels of natural and/or recombinant Human TNF alpha proteins.
LS-F24688 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human TNF Alpha in samples of Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates and Urine. It is based upon a Sandwich assay principle and can be used to detect levels of TNF Alpha as low as 1 picograms per milliliter.
All LSBio Custom kits have been predesigned and all necessary components, including antibodies and standards have been identified. Upon receiving a custom kit order, the kit will be assembled and quality control tested before being shipped out. Kit assembly and testing typically takes 4 to 6 weeks. In most cases the final kit is based on the Sandwich assay principle, with a few being Competitive EIA based. Specifications such as Range, Sensitivity, and Precision are defined upon completion. In the event that the custom kit cannot be successfully developed with 6 weeks of the order date, the customer will be notified and the order canceled at no cost.
Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates, Urine
96-Well Strip Plate
Colorimetric - 450nm
15.6 - 1000 pg/ml
1 pg/ml
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping. ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Coated 96-well Strip Plate
  • Standard (Lyophilized)
  • Biotinylated Detection Antibody (100x)
  • Avidin-Biotin-HRP Complex (100x)
  • Sample Diluent
  • Biotinylated Detection Antibody Diluent
  • Avidin-Biotin-HRP Complex Diluent
  • Wash Buffer (20x)
  • TMB Substrate
  • Stop Solution
  • Adhesive Plate Sealers
    Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.2
Store at 4°C for 6 months or at -20°C for one year. Avoid freeze/thaw cycles.
For research use only.

About TNF Alpha

P01375 NM_000594 NP_000585.2

TNF Elisa Kit, APC1 protein Elisa Kit, Cachectin Elisa Kit, DIF Elisa Kit, TNF Alpha Elisa Kit, Tumor necrosis factor-alpha Elisa Kit, TNF-alpha Elisa Kit, TNF-a Elisa Kit, TNFA Elisa Kit, Tumor necrosis factor Elisa Kit, TNF, macrophage-derived Elisa Kit, TNF, monocyte-derived Elisa Kit, TNFSF2 Elisa Kit

TNF Alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.

Requested From: United States
Date Requested: 10/27/2016

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