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Human ProGRP / Pro-Gastrin-Releasing Peptide ELISA Kit (Sandwich ELISA) - LS-F22987

1 plate
Available Discounts
5% off 5+ units

Product Description

LS-F22987 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human ProGRP / Pro-Gastrin-Releasing Peptide in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of ProGRP / Pro-Gastrin-Releasing Peptide as low as 9.38 picograms per milliliter.


Sandwich ELISA (enzyme-linked immunosorbent assay) kit
ProGRP / Pro-Gastrin-Releasing Peptide
This kit recognizes natural and recombinant Human ProGRP.
Intended Sample Types
Plasma, Serum
96-Well Microplate
Colorimetric - 450nm (TMB)
Detection Range
15.63 - 1000 pg/ml
9.38 pg/ml
Assay Time
3.5 h
Intra-Assay: CV<5.28% Inter-Assay: CV<5.52%
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • User Manual
  • 96-well Microplate
  • Detection Reagents
  • Wash Buffer
  • Substrate
  • Stop Solution
  • Adhesive Plate Sealers
  1. Prepare all reagents, samples and standards.
  2. Add 100 µl standard or sample to each well and incubate for 90 minutes at 37°C.
  3. Aspirate and add 100 µl prepared Detection Antibody and incubate of 1 hour at 37°C.
  4. Aspirate and wash 3 times.
  5. Add 100 µl HRP Conjugate and incubate for 30 minutes at 37°C.
  6. Aspirate and wash 5 times.
  7. Add 90 µl Substrate Solution and incubate for 15-20 minutes at 37°C.
  8. Add 50 µl Stop Solution.
  9. Read immediately at 450nm.
For research use only.

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Requested From: United States
Date Requested: 7/19/2019
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