LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
Human Phospho-TOP2A / Topoisomerase II Alpha (Thr1343) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1709
LS-F1709 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human TOP2A / Topoisomerase II Alpha in samples of Adherent Cell Cultures. It is based upon a Cell-Based Phosphorylation assay principle.
Catalog
Size
Price
LS-F1709-1
1 plate
$590

Popular TOP2A / Topoisomerase II Alpha Elisa Kits

Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 0.312-20 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma
Type: Cell-Based Phosphorylation ELISA
Format: 96-Well Microplate
Reactivity: Human
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures
Type: Cell-Based Phosphorylation ELISA
Format: 96-Well Microplate
Reactivity: Mouse, Human, Rat
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures
Type: Cell-Based ELISA
Format: 96-Well Microplate
Reactivity: Human
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures

Product Description

LS-F1709 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human TOP2A / Topoisomerase II Alpha in samples of Adherent Cell Cultures. It is based upon a Cell-Based Phosphorylation assay principle.
About TOP2A / Topoisomerase II Alpha
TOP2A / Topoisomerase II Alpha is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. P11388 NM_001067 NP_001058.2

TOP2A ElisaKit, DNA topoisomerase II, 170 kD ElisaKit, TOP2 ElisaKit, DNA gyrase ElisaKit, DNA topoisomerase 2-alpha ElisaKit, TP2A ElisaKit


Specifications

Type
Cell-Based Phoshorylation ELISA (enzyme-linked immunosorbent assay) kit
Target
TOP2A / Topoisomerase II Alpha
Reactivity
Human
Specificity
TOP2A (Phospho-Thr1343)
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • 96-Well Cell Culture Clear-Bottom Microplate
  • 10x TBS
  • Quenching Buffer
  • Blocking Buffer
  • 10x Wash Buffer
  • 100x Anti-TOP2A (Phospho-Thr1343) Antibody (Rabbit Polyclonal)
  • 100x Anti-TOP2A Antibody (Rabbit Polyclonal)
  • 100x Anti-GAPDH Antibody (Mouse Monoclonal)
  • HRP-Conjugated Anti-Rabbit IgG Antibody
  • HRP-Conjugated Anti-Mouse IgG Antibody
  • Primary Antibody Diluent
  • Ready-to-Use Substrate
  • Stop Solution
  • Crystal Violet Solution
  • SDS Solution
  • Adhesive Plate Seals
Protocol
                Restrictions
                For research use only.
                Guarantee
                This elisa kit carries the LSBio 100% Guarantee.

                Publications (0)


                Customer Reviews (0)


                Images

                Cell-Based Phosphorylation ELISA Platform Overview
                Cell-Based Phosphorylation ELISA Platform Overview

                Cell-Based Phosphorylation ELISA Platform Overview
                Cell-Based Phosphorylation ELISA Platform Overview

                Cell-Based Phosphorylation ELISA Platform Overview
                Cell-Based Phosphorylation ELISA Platform Overview

                Cell-Based Phosphorylation ELISA Platform Overview
                Cell-Based Phosphorylation ELISA Platform Overview

                Requested From: United States
                Date Requested: 8/21/2018

                Get Social With Us!
                Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
                Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy