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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Human Phospho-KRT18 / CK18 / Cytokeratin 18 (Ser52) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1344
LS-F1344 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human KRT18 / CK18 / Cytokeratin 18 in samples of Adherent Cell Cultures. It is based upon a Cell-Based Phosphorylation assay principle.
Catalog
Size
Price
LS-F1344-1
1 plate
$590

Popular KRT18 / CK18 / Cytokeratin 18 Elisa Kits

Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 1.56-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Tissue Homogenates, Plasma
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Rat
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Cell Lysates, Serum, Tissue Homogenates, Plasma
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Mouse
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Tissue Homogenates, Plasma
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 62.5-4000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma
Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
Reactivity: Rat
Range: 0.16-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

Product Description

LS-F1344 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human KRT18 / CK18 / Cytokeratin 18 in samples of Adherent Cell Cultures. It is based upon a Cell-Based Phosphorylation assay principle.
About KRT18 / CK18 / Cytokeratin 18
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. P05783 NM_000224 NP_000215.1

KRT18 ElisaKit, CK-18 ElisaKit, Cytokeratin 18 ElisaKit, Cytokeratin-18 ElisaKit, K18 ElisaKit, Keratin 18 ElisaKit, CYK18 ElisaKit, Keratin-18 ElisaKit


Specifications

Type
Cell-Based Phoshorylation ELISA (enzyme-linked immunosorbent assay) kit
Target
KRT18 / CK18 / Cytokeratin 18
Reactivity
Human
Specificity
Keratin 18 (Phospho-Ser52)
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • 96-Well Cell Culture Clear-Bottom Microplate
  • 10x TBS
  • Quenching Buffer
  • Blocking Buffer
  • 10x Wash Buffer
  • 100x Anti-Keratin 18 (Phospho-Ser52) Antibody (Rabbit Polyclonal)
  • 100x Anti-Keratin 18 Antibody (Rabbit Polyclonal)
  • 100x Anti-GAPDH Antibody (Mouse Monoclonal)
  • HRP-Conjugated Anti-Rabbit IgG Antibody
  • HRP-Conjugated Anti-Mouse IgG Antibody
  • Primary Antibody Diluent
  • Ready-to-Use Substrate
  • Stop Solution
  • Crystal Violet Solution
  • SDS Solution
  • Adhesive Plate Seals
Protocol
                Restrictions
                For research use only.
                Guarantee
                This elisa kit carries the LSBio 100% Guarantee.

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                Images

                Cell-Based Phosphorylation ELISA Platform Overview
                Cell-Based Phosphorylation ELISA Platform Overview

                Cell-Based Phosphorylation ELISA Platform Overview
                Cell-Based Phosphorylation ELISA Platform Overview

                Cell-Based Phosphorylation ELISA Platform Overview
                Cell-Based Phosphorylation ELISA Platform Overview

                Cell-Based Phosphorylation ELISA Platform Overview
                Cell-Based Phosphorylation ELISA Platform Overview

                Requested From: United States
                Date Requested: 8/15/2018

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