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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Human PDGFRA / PDGFR Alpha ELISA Kit (Sandwich ELISA) - LS-F3379
Catalog
Size
Price
LS-F3379-1
1 plate
$470
Available Discounts
10% off 2-4 units
20% off 5+ units

Popular PDGFRA / PDGFR Alpha Elisa Kits


Product Description

LS-F3379 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human PDGFRA / PDGFR Alpha in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of PDGFRA / PDGFR Alpha as low as 0.492 nanograms per millilter.

Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
PDGFRA / PDGFR Alpha
Synonyms
PDGFRA, CD140A, CD140a antigen, PDGFalphaR, PDGFR2, PDGFRalpha, AlphaPDGFR, RHEPDGFRA, PDGF receptor alpha, PDGFR-2, PDGFRA/BCR fusion, APDGFR, Pdgf alpha-receptor, PDGF-R-alpha, PDGFR-alpha
Reactivity
Human
Manual
Intended Sample Types
Cell Culture Supernatants, Plasma, Serum
Sample Dilution
2 fold
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.492 - 48 ng/ml
Sensitivity
0.492 ng/ml
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Coated 96-well Strip Plate
  • Wash Buffer Concentrate (20X)
  • Standard (Lyophilized)
  • Biotinylated Detection Antibody
  • HRP-Streptavidin Conjugate (500x)
  • TMB One-Step Substrate Reagent
  • Stop Solution
  • Assay Diluent C
  • Assay Diluent B (5x)
  • Adhesive Plate Sealers
Protocol
                        Restrictions
                        For research use only.
                        About PDGFRA / PDGFR Alpha
                        Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. P16234 NM_006206 NP_006197.1


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                        Sandwich ELISA Platform Overview
                        Sandwich ELISA Platform Overview

                        Sandwich ELISA Platform Overview
                        Sandwich ELISA Platform Overview

                        Sandwich ELISA Platform Overview
                        Sandwich ELISA Platform Overview

                        Sandwich ELISA Platform Overview
                        Sandwich ELISA Platform Overview

                        Requested From: United States
                        Date Requested: 10/21/2018

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