Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
LS-F2890 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Osteonectin / SPARC in samples of Cell Culture Supernatants, Milk and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of Osteonectin / SPARC as low as 20 nanograms per millilter.
SPARC, Basement-membrane protein 40, BM-40, Cysteine-rich protein, ON, Osteonectin
This kit is for the detection of natural and recombinant human SPARC. No significant cross-reactivity or interference between SPARC and analogs was observed.
Intended Sample Types
Cell Culture Supernatants, Milk, Serum
Colorimetric - 450nm (TMB)
0.78 - 50 ng/ml
Intra-Assay: CV%<5.1% Inter-Assay: CV%<7.4%
Short term: 4°C; Long term: see manual.
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
SPARC (secreted protein acidic and rich in cysteine)/Osteonectin is a matricellular glycoprotein that modulates cellular interactions with the ECM and is expressed in tissues undergoing remodeling. It functions as a de-adhesive protein, as a modulator of growth factor activity, and as a cell-cycle inhibitor. It induces changes in endothelial cell shape via F-actin, coincident with the appearance of intercellular gaps, that provide a paracellular pathway for extravasation of macromolecules.