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Human NPY2R ELISA Kit (Sandwich ELISA) - LS-F36757

Catalog Size Price
LS-F36757-1 1 plate Unavailable

Most Popular NPY2R Elisa Kits

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100% Guaranteed
LS-F36757
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
NPY2R
Human
LS-F36757 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human NPY2R in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of NPY2R as low as 0.188 nanograms per millilter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
0.313 - 20 ng/ml
0.188
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- NPYR2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- NPYR2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NPYR2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NPYR2 can be calculated.
Short term: 4°C; Long term: see manual.
For research use only.

About NPY2R

P49146 U36269 NP_000901.1

NPY2R ElisaKit, Neuropeptide Y2 receptor ElisaKit, NPY-Y2 receptor ElisaKit, Neuropeptide Y receptor Y2 ElisaKit, Neuropeptide y-y2 ElisaKit, NPY2-R ElisaKit, Type 2 neuropeptide y receptor ElisaKit, Hypothalamic Y2 receptor ElisaKit, Neuropeptide Y receptor type 2 ElisaKit, Peptide yy y2 receptor ElisaKit, Y2 receptor ElisaKit

Receptor for neuropeptide Y and peptide YY. The rank order of affinity of this receptor for pancreatic polypeptides is PYY > NPY > PYY (3-36) > NPY (2-36) > [Ile-31, Gln-34] PP > [Leu-31, Pro-34] NPY > PP, [Pro-34] PYY and NPY free acid.

 

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: 
Date Requested: 5/23/2018

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