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Human MYLK ELISA Kit (Sandwich ELISA) - LS-F36654

Catalog Size Price
LS-F36654-1 1 plate Unavailable

Most Popular MYLK Elisa Kits

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100% Guaranteed
LS-F36654
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
MYLK
Human
LS-F36654 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human MYLK in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of MYLK as low as 0.094 nanograms per millilter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
0.156 - 10 ng/ml
0.094
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MLCK/MYLK antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- MLCK/MYLK antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MLCK/MYLK amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MLCK/MYLK can be calculated.
Short term: 4°C; Long term: see manual.
For research use only.

About MYLK

Q15746 NM_053025 NP_444253.3

MYLK ElisaKit, AAT7 ElisaKit, KRP ElisaKit, MLCK ElisaKit, MLCK108 ElisaKit, Kinase-related protein ElisaKit, MLCK1 ElisaKit, Telokin ElisaKit, MLC kinase ElisaKit, MLCK210 ElisaKit, MSTP083 ElisaKit, MYLK1 ElisaKit, Myosin light chain kinase ElisaKit, SmMLCK ElisaKit

Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma.

 

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: 
Date Requested: 5/24/2018

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