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Human MYC / c-Myc ELISA Kit (Sandwich ELISA) - LS-F36240

Catalog Size Price
LS-F36240-1 1 plate Unavailable

Most Popular MYC / c-Myc Elisa Kits

Mouse/Human/Rat MYC / c-Myc ELISA Kit (DNA-Binding ELISA) - LS-F786
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12 x 8-Well Microstrips
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Mouse/Human Phospho-MYC / c-Myc (Ser62) ELISA Kit (DNA-Binding Phosphorylation ELISA) - LS-F886
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12 x 8-Well Microstrips
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Human MYC / c-Myc ELISA Kit (Sandwich ELISA) - LS-F4532
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1.56-100 ng/ml
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Mouse MYC / c-Myc ELISA Kit (Sandwich ELISA) - LS-F16761
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Human MYC / c-Myc ELISA Kit (Sandwich ELISA) - LS-F21005
Quantitative Sandwich ELISA
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100% Guaranteed
LS-F36240
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
MYC / c-Myc
Human
LS-F36240 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human MYC / c-Myc in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of MYC / c-Myc as low as 0.188 nanograms per millilter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
0.313 - 20 ng/ml
0.188
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- c-myc antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- c-myc antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the c-myc amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of c-myc can be calculated.
Short term: 4°C; Long term: see manual.
For research use only.

About MYC / c-Myc

P01106 NM_002467 NP_002458.2

MYC ElisaKit, BHLHe39 ElisaKit, C-Myc ElisaKit, MRTL ElisaKit, Myc proto-oncogene protein ElisaKit, Proto-oncogene c-Myc ElisaKit, Rats1 ElisaKit, Transcription factor p64 ElisaKit, v-myc ElisaKit

MYC / c-Myc is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.

 

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: 
Date Requested: 5/27/2018

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