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Human MTPAP ELISA Kit (Competitive EIA) - LS-F39409
Catalog
Size
Price
LS-F39409-1
1 plate
$570

Popular MTPAP Elisa Kits


Product Description

LS-F39409 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human MTPAP in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Competitive EIA assay principle and can be used to detect levels of MTPAP as low as 0.188 nanograms per millilter.

Specifications

Type
Competitive EIA (enzyme immunoassay) kit
Target
MTPAP
Synonyms
MTPAP, PAP, Terminal uridylyltransferase 1, PAPD1, TUTase 1, RP11-305E6.3, SPAX4
Reactivity
Human
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.312 - 20 ng/ml
Sensitivity
0.188 ng/ml
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
Store at 4°C. Stable for 6 months.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP Streptavidin Conjugate Diluent
  • Sample Diluent
  • Standard (Lyophilized)
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Adhesive Plate Sealers
Background
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with MTPAP. During the reaction, MTPAP in the sample or standard competes with a fixed amount of MTPAP on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to MTPAP. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MTPAP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions
For research use only.
About MTPAP
Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA. Q9NVV4 NM_018109 NP_060579.3


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Competition ELISA Platform Overview
Competition ELISA Platform Overview

Competition ELISA Platform Overview
Competition ELISA Platform Overview

Competition ELISA Platform Overview
Competition ELISA Platform Overview

Competition ELISA Platform Overview
Competition ELISA Platform Overview

Requested From: United States
Date Requested: 10/17/2018

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