Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
This assay has high sensitivity and excellent specificity for detection of human MMP-9. No significant cross-reactivity or interference between human MMP-9 and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human MMP-9 and all the analogs, therefore, cross reaction may still exist.
LS-F10064 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human MMP9 / Gelatinase B in samples of Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates and Urine. It is based upon a Sandwich assay principle and can be used to detect levels of MMP9 / Gelatinase B as low as 0.284 nanograms per millilter.
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Assay plate (12 x 8 coated Microwells)
Biotin-antibody (100 x concentrate)
HRP-avidin (100 x concentrate)
Wash Buffer (25 x concentrate)
Adhesive Strip (For 96 wells)
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
MMP9 ElisaKit, 92kd gelatinase ElisaKit, 92 kd type iv collagenase ElisaKit, 92 kDa gelatinase ElisaKit, 92 kDa type IV collagenase ElisaKit, Matrix metalloproteinase-9 ElisaKit, MMP-9 ElisaKit, Type V collagenase ElisaKit, CLG4B ElisaKit, Gelatinase B ElisaKit, GELB ElisaKit, Macrophage gelatinase ElisaKit, MANDP2 ElisaKit
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens.