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Human IL1RN ELISA Kit (Sandwich ELISA) - LS-F36623

Catalog Size Price
LS-F36623-1 1 plate Unavailable

Most Popular IL1RN Elisa Kits

Bovine IL1RN ELISA Kit (Sandwich ELISA) - LS-F6096
Sandwich ELISA
96-Well Strip Plate
Bovine
31.25-2000 pg/ml
Colorimetric - 450nm (TMB)
Serum, Tissue Homogenates, Plasma
Human IL1RN ELISA Kit (Sandwich ELISA) - LS-F11764
Quantitative Sandwich ELISA
96-Well Strip Plate
Human
0.156-10 ng/ml
Colorimetric - 450nm (TMB)
Serum, Plasma
Rat IL1RN ELISA Kit (Sandwich ELISA) - LS-F11765
Quantitative Sandwich ELISA
96-Well Strip Plate
Rat
31.2-2000 pg/ml
Colorimetric - 450nm (TMB)
Serum, Plasma
Human IL1RN ELISA Kit (Sandwich ELISA) - LS-F24563
Quantitative Sandwich ELISA
96-Well Strip Plate
Human
31.2-2000 pg/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Serum, Plasma
Mouse IL1RN ELISA Kit (Sandwich ELISA) - LS-F24841
Quantitative Sandwich ELISA
96-Well Strip Plate
Mouse
62.5-4000 pg/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Serum, Plasma

100% Guaranteed
LS-F36623
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
IL1RN
Human
LS-F36623 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human IL1RN in samples of Plasma and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of IL1RN as low as 18.75 picograms per milliliter.
Plasma, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
31.25 - 2000 pg/ml
18.75
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL- 1ra/IL-1F3antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IL- 1ra/IL-1F3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-1ra/IL-1F3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL- 1ra/IL-1F3 can be calculated.
Short term: 4°C; Long term: see manual.
For research use only.

About IL1RN

P18510 NM_000577 NP_000568.1

IL1RN ElisaKit, Anakinra ElisaKit, DIRA ElisaKit, IL-1ra ElisaKit, IL-1RN ElisaKit, IL1F3 ElisaKit, IL1RA ElisaKit, MVCD4 ElisaKit, IL1 inhibitor ElisaKit, IL1RN (IL1F3) ElisaKit, IRAP ElisaKit, ICIL-1RA ElisaKit, IL-1ra3 ElisaKit

IL1RN is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer.

 

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: 
Date Requested: 5/20/2018

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