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Human IL19 ELISA Kit (Sandwich ELISA) - LS-F36771

Catalog Size Price
LS-F36771-1 1 plate Unavailable

Most Popular IL19 Elisa Kits

Human IL19 ELISA Kit (Sandwich ELISA) - LS-F642
Sandwich ELISA
12 x 8-Well Microstrips
Human
32-2000 pg/ml
Colorimetric - 450nm (TMB)
Cell Lysates, Serum, Plasma
Rat IL19 ELISA Kit (Sandwich ELISA) - LS-F4756
Sandwich ELISA
96-Well Strip Plate
Rat
15.63-1000 pg/ml
Colorimetric - 450nm (TMB)
Serum, Plasma
Mouse IL19 ELISA Kit (Sandwich ELISA) - LS-F6309
Sandwich ELISA
96-Well Strip Plate
Mouse
7.8-500 pg/ml
Colorimetric - 450nm (TMB)
Serum, Plasma
Human IL19 ELISA Kit (Sandwich ELISA) - LS-F6670
Sandwich ELISA
96-Well Strip Plate
Human
15.63-1000 pg/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Cell Lysates, Serum, Tissue Homogenates, Plasma
Mouse IL19 ELISA Kit (Custom ELISA) - LS-F19215
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96-Well Strip Plate
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100% Guaranteed
LS-F36771
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
IL19
Human
LS-F36771 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human IL19 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of IL19 as low as 18.75 picograms per milliliter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
31.25 - 2000 pg/ml
18.75
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-19 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IL-19 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-19 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-19 can be calculated.
Short term: 4°C; Long term: see manual.
For research use only.

About IL19

Q9UHD0 NM_013371 NP_037503.2

IL19 ElisaKit, MDA1 ElisaKit, IL-19 ElisaKit, Interleukin 19 ElisaKit, ZMDA1 ElisaKit, IL-10C ElisaKit, Interleukin-19 ElisaKit, NG.1 ElisaKit

IL19 is a cytokine that belongs to the IL10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses.

 

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: 
Date Requested: 5/23/2018

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