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Human IL12B / IL12 p40 ELISA Kit (Sandwich ELISA) - LS-F24548

Ordering

Size Price
1 plate $550
Inquire for larger quantities
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LS-F24548
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
IL12B / IL12 p40
Human
For the detection and quantification of endogenous levels of natural and/or recombinant Human IL-12 p40 proteins.
LS-F24548 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human IL12B / IL12 p40 in samples of Cell Culture Supernatants, Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of IL12B / IL12 p40 as low as 30 picograms per milliliter.
All LSBio Custom kits have been predesigned and all necessary components, including antibodies and standards have been identified. Upon receiving a custom kit order, the kit will be assembled and quality control tested before being shipped out. Kit assembly and testing typically takes 4 to 6 weeks. In most cases the final kit is based on the Sandwich assay principle, with a few being Competitive EIA based. Specifications such as Range, Sensitivity, and Precision are defined upon completion. In the event that the custom kit cannot be successfully developed with 6 weeks of the order date, the customer will be notified and the order canceled at no cost.
Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm
Quantitative
62.5 - 4000 pg/ml
30 pg/ml
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping. ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Coated 96-well Strip Plate
  • Standard (Lyophilized)
  • Biotinylated Detection Antibody (100x)
  • Avidin-Biotin-HRP Complex (100x)
  • Sample Diluent
  • Biotinylated Detection Antibody Diluent
  • Avidin-Biotin-HRP Complex Diluent
  • Wash Buffer (20x)
  • TMB Substrate
  • Stop Solution
  • Adhesive Plate Sealers
    Interleukin-12 (IL12; formerly NKSF, for natural killer cell stimulatory factor, or CLMF, for cytotoxic lymphocyte maturation factor) is a novel cytokine cloned from B-cell lines. IL-12 is a heterodimeric molecule composed of p35 and p40 subunits. The larger 40-kDa subunit (p40) is a member of the cytokine receptor family, and the smaller 35-kDa subunit (p35) is related to IL6 and GCSF. Both IL-12 p40 (-/-) and p35 (-/-) mice fail to produce IL-12 p70 heterodimer. Interleukin (IL)-12 has been cloned on the basis of its ability to activate natural killer (NK) cells and promote the development of cytolytic T cells. With further understanding of its activities, IL-12 has emerged as an important cytokine, affecting both immune and hematologic functions. It has been shown to be necessary for the T cell independent induction of interferon (IFN)-gamma, critical for the initial suppression of bacterial and parasitic infection; for the development of a Th1 response, critical for effective host defense against intracellular pathogens; and for the activation of differentiated T lymphocytes of both CD4+ and CD8+ phenotype. The subunits map to different chromosomes: p40 (IL12B) to 5q31-q33 and p35 (IL12A) to 3p12-3q13.2.
Store at 4°C for 6 months or at -20°C for one year. Avoid freeze/thaw cycles.
For research use only.

About IL12B / IL12 p40

P29460 NM_002187 NP_002178.2

IL12B Elisa Kit, CLMF p40 Elisa Kit, IL-12 p40 Elisa Kit, IL-12 subunit p40 Elisa Kit, Interleukin-12 beta chain Elisa Kit, Interleukin-12 subunit beta Elisa Kit, IL12, subunit p40 Elisa Kit, Interleukinl-12 Elisa Kit, Interleukin 12, p40 Elisa Kit, NKSF Elisa Kit, CLMF Elisa Kit, CLMF2 Elisa Kit, IL-12B Elisa Kit, NKSF2 Elisa Kit

IL12B / IL12 p40 is a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development.

Requested From: United States
Date Requested: 12/5/2016

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