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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Human IDE ELISA Kit (Sandwich ELISA) - LS-F23775
Catalog
Size
Price
LS-F23775-1
1 plate
$590
Available Discounts
5% off 5+ units

Popular IDE Elisa Kits

Reactivity: Human
Range: 0.313-20 ng/ml
Reactivity: Rat
Range: 0.313-20 ng/ml
Reactivity: Mouse
Range: 0.156-10 ng/ml
Reactivity: Mouse
Range: 0.312-20 ng/ml

Product Description

LS-F23775 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human IDE in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of IDE as low as 62.5 picograms per milliliter.

Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
IDE
Synonyms
IDE, Abeta-degrading protease, Insulinase, INSULYSIN, Insulin protease, Insulin-degrading enzyme
Reactivity
Human
Specificity
This kit recognizes natural and recombinant Human IDE.
Manual
Intended Sample Types
Plasma, Serum
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
62.5 - 4000 pg/ml
Sensitivity
62.5 pg/ml
Assay Time
3.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<10%
Storage
Store at 4°C. Do not freeze.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • User Manual
  • 96-well Microplate
  • Detection Reagents
  • Wash Buffer
  • Substrate
  • Stop Solution
  • Adhesive Plate Sealers
Protocol
                    Restrictions
                    For research use only.
                    About IDE
                    IDE is a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. P14735 NM_004969 NP_004960.2


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                    Sandwich ELISA Platform Overview
                    Sandwich ELISA Platform Overview

                    Sandwich ELISA Platform Overview
                    Sandwich ELISA Platform Overview

                    Sandwich ELISA Platform Overview
                    Sandwich ELISA Platform Overview

                    Sandwich ELISA Platform Overview
                    Sandwich ELISA Platform Overview

                    Requested From: United States
                    Date Requested: 11/15/2018

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