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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Human ICAM-1 / CD54 ELISA Kit (Sandwich ELISA) - LS-F3339
LS-F3339 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human ICAM-1 / CD54 in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of ICAM-1 / CD54 as low as 0.15 nanograms per millilter.
Catalog
Size
Price
LS-F3339-1
1 plate
$470
Available Discounts
10% off 2-4 units
20% off 5+ units

Popular ICAM-1 / CD54 Elisa Kits

Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Rat
Range: 0.313-20 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Cell Lysates, Serum, Tissue Homogenates, Plasma
Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
Reactivity: Rat
Range: 20-7000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Serum, Plasma
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 78.13-5000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Cell Lysates, Serum, Tissue Homogenates, Plasma
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 0.781-50 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Tissue Homogenates, Plasma
Type: Cell-Based ELISA
Format: 96-Well Microplate
Reactivity: Human
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures

Product Description

LS-F3339 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human ICAM-1 / CD54 in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of ICAM-1 / CD54 as low as 0.15 nanograms per millilter.
About ICAM-1 / CD54
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. P05362 NM_000201 NP_000192.2

ICAM1 ElisaKit, BB2 ElisaKit, CD54 antigen ElisaKit, CD54 ElisaKit, ICAM-1 ElisaKit, p3.58 ElisaKit


Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
ICAM-1 / CD54
Reactivity
Human
Manual
Intended Sample Types
Cell Culture Supernatants, Plasma, Serum
Sample Dilution
5-200 fold
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.313 - 20 ng/ml
Sensitivity
0.15 ng/ml
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Coated 96-well Strip Plate
  • Wash Buffer Concentrate (20X)
  • Standard (Lyophilized)
  • Biotinylated Detection Antibody
  • HRP-Streptavidin Conjugate (340x)
  • TMB Substrate
  • Stop Solution
  • Assay Diluent A
  • Assay Diluent B (5x)
  • Adhesive Plate Sealers
Protocol
                        Restrictions
                        For research use only.
                        Guarantee
                        This elisa kit carries the LSBio 100% Guarantee.

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                        Sandwich ELISA Platform Overview
                        Sandwich ELISA Platform Overview

                        Sandwich ELISA Platform Overview
                        Sandwich ELISA Platform Overview

                        Sandwich ELISA Platform Overview
                        Sandwich ELISA Platform Overview

                        Requested From: United States
                        Date Requested: 8/19/2018

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