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Human Complement C5a ELISA Kit (Sandwich ELISA) - LS-F431

Catalog Size Price
LS-F431-1 1 plate Unavailable
Available Discounts 10% off 2-4 units
20% off 5+ units

Most Popular Complement C5a Elisa Kits

Human Complement C5a ELISA Kit (Sandwich ELISA) - LS-F431
Quantitative Sandwich ELISA
96-Well Microplate
Human
31.25-2000 pg/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Serum, Plasma
 Image
Mouse Complement C5a ELISA Kit (Sandwich ELISA) - LS-F2812
Quantitative Sandwich ELISA
96-Well Microplate
Mouse
15.6-1000 pg/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Serum, Plasma
 Image
Pig Complement C5a ELISA Kit (Sandwich ELISA) - LS-F21690
Quantitative Sandwich ELISA
96-Well Microplate
Pig
0.156-10 ng/ml
Colorimetric - 450nm (TMB)
Serum, Plasma
 Image
Human Complement C5a ELISA Kit (Sandwich ELISA) - LS-F23962
Quantitative Sandwich ELISA
96-Well Microplate
Human
78.125-5000 pg/ml
Colorimetric - 450nm (TMB)
Serum, Plasma
 Image
Mouse Complement C5a ELISA Kit (Sandwich ELISA) - LS-F24035
Quantitative Sandwich ELISA
96-Well Microplate
Mouse
62.5-4000 pg/ml
Colorimetric - 450nm (TMB)
Serum, Plasma
 Image

100% Guaranteed
LS-F431
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Complement C5a
Human
The antibody pair provided in this kit recognizes human Complement C5a. Antibodies in this kit show 50% cross reactivity with C5, No cross reactivity with recombinant human C3a, mouse C5a, mouse C5d or human alpha-2-macrogolbulin.
LS-F431 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Complement C5a in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of Complement C5a as low as 31.25 picograms per milliliter.
Cell Culture Supernatants, Plasma, Serum
40-400 fold
96-Well Microplate
Colorimetric - 450nm (TMB)
Quantitative
31.25 - 2000 pg/ml
31.25 pg/ml
Intra-Assay: CV<10% Inter-Assay: CV<12%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Coated 96-well Strip Plate
  • Wash Buffer Concentrate (20X)
  • Standard (Lyophilized)
  • Biotinylated Detection Antibody
  • HRP-Streptavidin Conjugate (200x)
  • TMB Substrate
  • Stop Solution
  • Assay Diluent A
  • Assay Diluent B (5x)
  • Adhesive Plate Sealers
May be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standard should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze/thaw cycles.
For research use only.

About Complement C5a

C5a ElisaKit

C5a is a protein fragment released from cleavage complement component C5 by protease C5-convertase into C5a and C5b fragments. C5b is important in late events of complement cascade, whereas C5a acts as highly inflammatory peptide. The origin of C5 is in the hepatocyte but its synthesis can also be found in macrophages that may cause local increase of C5a. C5a has chemotactic and anaphylatoxic properties, it is essential in the innate immunity but it is also linked with the adaptive immunity.

 

Sandwich ELISA Platform Overview

Requested From: 
Date Requested: 10/23/2017

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