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Human CNPase ELISA Kit (Sandwich ELISA) - LS-F39177

Catalog Size Price
LS-F39177-1 1 plate Unavailable

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Human CNPase ELISA Kit (Sandwich ELISA) - LS-F39177
Quantitative Sandwich ELISA
96-Well Strip Plate
Human
0.312-20 ng/ml
Colorimetric - 450nm (TMB)
Serum, Tissue Homogenates, Plasma

100% Guaranteed
LS-F39177
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
CNPase
Human
LS-F39177 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CNPase in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CNPase as low as 0.188 nanograms per millilter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
0.312 - 20 ng/ml
0.188
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP Streptavidin Conjugate Diluent
  • Sample Diluent
  • Standard (Lyophilized)
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Adhesive Plate Sealers
    This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with CNP. During the reaction, CNP in the sample or standard competes with a fixed amount of CNP on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to CNP. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CNP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Short term: 4°C; Long term: see manual.
For research use only.

About CNPase

P09543 NM_033133 NP_149124.3

CNP ElisaKit, CNP1 ElisaKit, CNPase ElisaKit, Phosphodiesterase CNP ElisaKit

May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin.

 

Requested From: 
Date Requested: 5/24/2018

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