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LS-F36184 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CENPJ / LAP in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CENPJ / LAP as low as 0.469 nanograms per millilter.
CENPJ, BM032, Centromere protein J, CENP-J, LAP, LYST-interacting protein 1, LIP1, LYST-interacting protein LIP7, SASS4, Sas-4, LYST-interacting protein LIP1, CPAP, LAG-3-associated protein, MCPH6, SCKL4
Intended Sample Types
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
0.781 - 50 ng/ml
Intra-Assay: CV<8% Inter-Assay: CV<10%
Store at 4°C. Stable for 6 months.
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Adhesive Plate Sealers
Coated 96-well Strip Plate
Detection Antibody Diluent
Biotinylated Detection Antibody (100x)
HRP-Streptavidin Conjugate (100x)
HRP-Streptavidin Conjugate Diluent
Wash Buffer (25x)
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- LAPantibody was pre-coated onto 96-well plates. And the biotin conjugated anti- LAP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the LAP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of LAP can be calculated.
CENPJ / LAP is a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein.