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Human CDKN2A ELISA Kit (Sandwich ELISA) - LS-F22610
Catalog
Size
Price
LS-F22610-1
1 plate
$590
Available Discounts
5% off 5+ units

Popular CDKN2A Elisa Kits

Reactivity: Rat
Range: 0.156-10 ng/ml
Reactivity: Human
Range: 1.56-100 ng/ml
Reactivity: Human
Range: 2.5-50 ng/ml
Reactivity: Human
Range: 125-8000 pg/ml

Product Description

LS-F22610 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CDKN2A in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of CDKN2A as low as 0.625 nanograms per millilter.

Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
CDKN2A
Synonyms
CDKN2A, ARF, Cdk4 inhibitor, CDK4 inhibitor p16-INK4, INK4, INK4A, Multiple tumor suppressor 1, MTS-1, MTS1, p16-INK4, p16-INK4A, p16INK4, p19, p14, p16, p16 INK4A, p16INK4A, p14ARF, TP16, CDK4I, CDKN2, CMM2, MLM, p19ARF
Reactivity
Human
Specificity
This kit recognizes natural and recombinant CDKN2A.
Manual
Intended Sample Types
Plasma, Serum
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.625 - 40 ng/ml
Sensitivity
0.625 ng/ml
Assay Time
3.5 h
Precision
Intra-Assay: CV<5.89% Inter-Assay: CV<5.73%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Coated 96-well Strip Plate
  • Standard (Lyophilized)
  • Sample Diluent
  • Biotinylated Detection Antibody (100x)
  • Biotinylated Detection Antibody Diluent
  • HRP Conjugate (100x)
  • HRP Conjugate Diluent
  • Wash Buffer (25x)
  • TMB Substrate
  • Stop Solution
  • Adhesive Plate Sealers
Protocol
                    Restrictions
                    For research use only.
                    About CDKN2A
                    Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. P42771 NM_000077 NP_000068.1


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                    Sandwich ELISA Platform Overview

                    Requested From: United States
                    Date Requested: 10/21/2018

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