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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Human CD30L / CD153 ELISA Kit (Sandwich ELISA) - LS-F36230
LS-F36230 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CD30L / CD153 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CD30L / CD153 as low as 4.688 picograms per milliliter.
Catalog
Size
Price
LS-F36230-1
1 plate
$790

Popular CD30L / CD153 Elisa Kits

Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
Reactivity: Mouse
Range: 0.41-40 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Serum, Plasma
Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
Reactivity: Mouse
Range: 15.6-1000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Serum, Plasma
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 78.13-5000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Tissue Homogenates, Plasma
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 15.6-1000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma
Type: Custom ELISA
Format: 96-Well Strip Plate
Reactivity: Mouse
Detection: Colorimetric - 450nm (TMB)

Product Description

LS-F36230 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CD30L / CD153 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CD30L / CD153 as low as 4.688 picograms per milliliter.
About CD30L / CD153
CD30L / CD153 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. P32971 NM_001244 NP_001235.1

TNFSF8 ElisaKit, CD153 ElisaKit, CD30 antigen ligand ElisaKit, CD30-L ElisaKit, CD30L ElisaKit, CD30LG ElisaKit, CD153 antigen ElisaKit, CD30 ligand ElisaKit


Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
CD30L / CD153
Reactivity
Human
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
7.813 - 500 pg/ml
Sensitivity
4.688
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
Background
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CD30L antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CD30L antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CD30L amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CD30L can be calculated.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.

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Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 8/15/2018

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