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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Human CCND3 / Cyclin D3 ELISA Kit (Sandwich ELISA) - LS-F38967
LS-F38967 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CCND3 / Cyclin D3 in samples of Cell Culture Supernatants, Cell Lysates and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CCND3 / Cyclin D3 as low as 0.056 nanograms per millilter.
Catalog
Size
Price
LS-F38967-1
1 plate
$670
Available Discounts
10% off 5+ units

Popular CCND3 / Cyclin D3 Elisa Kits

Type: Cell-Based ELISA
Format: 96-Well Microplate
Reactivity: Mouse, Human, Rat
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 78-5000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma
Type: Cell-Based Phosphorylation ELISA
Format: 96-Well Microplate
Reactivity: Mouse, Human, Rat
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures
Type: Custom ELISA
Format: 96-Well Strip Plate
Reactivity: Mouse
Detection: Colorimetric - 450nm (TMB)
Type: Custom ELISA
Format: 96-Well Strip Plate
Detection: Colorimetric - 450nm (TMB)

Product Description

LS-F38967 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CCND3 / Cyclin D3 in samples of Cell Culture Supernatants, Cell Lysates and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CCND3 / Cyclin D3 as low as 0.056 nanograms per millilter.
About CCND3 / Cyclin D3
Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. P30281 NM_001760 NP_001751.1

CCND3 ElisaKit, D3-type cyclin ElisaKit, G1/S-specific cyclin D3 ElisaKit, G1/S-specific cyclin-D3 ElisaKit, Cyclin D3 ElisaKit


Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
CCND3 / Cyclin D3
Reactivity
Human
Manual
Intended Sample Types
Cell Culture Supernatants, Cell Lysates, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.156 - 10 ng/ml
Sensitivity
0.056
Assay Time
4.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Coated 96-well Strip Plate
  • Adhesive Plate Sealers
  • Sample Diluent
  • Assay Diluent A
  • Assay Diluent B
  • Wash Buffer (30x)
  • TMB Substrate
  • Stop Solution
  • Standard (Lyophilized)
  • Detection Reagent A (Green)
  • Detection Reagent B (Red)
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.

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Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 8/21/2018

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