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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Human CASP8 / Caspase 8 ELISA Kit (Sandwich ELISA) - LS-F39693
LS-F39693 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CASP8 / Caspase 8 in samples of Cell Culture Supernatants and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of CASP8 / Caspase 8 as low as 10 picograms per milliliter.
Catalog
Size
Price
LS-F39693-1
1 plate
$530

Popular CASP8 / Caspase 8 Elisa Kits

Type: Cell-Based Phosphorylation ELISA
Format: 96-Well Microplate
Reactivity: Human, Rat
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures
Type: Cell-Based ELISA
Format: 96-Well Microplate
Reactivity: Mouse, Human, Rat
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Rat
Range: 0.313-20 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Lysates, Tissue Homogenates
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Mouse
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

Product Description

LS-F39693 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CASP8 / Caspase 8 in samples of Cell Culture Supernatants and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of CASP8 / Caspase 8 as low as 10 picograms per milliliter.
About CASP8 / Caspase 8
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Q14790 nm_001228 AAC50645.1

CASP8 ElisaKit, ALPS2B ElisaKit, Apoptotic protease Mch-5 ElisaKit, Apoptotic cysteine protease ElisaKit, Casp-8 ElisaKit, Caspase-8 ElisaKit, CAP4 ElisaKit, Caspase 8 ElisaKit, MACH ElisaKit, MCH5 ElisaKit, MORT1-associated ced-3 homolog ElisaKit, ICE-like apoptotic protease 5 ElisaKit, Pro Caspase 8 ElisaKit, FADD-like ICE ElisaKit, FLICE ElisaKit, MACH-alpha-1/2/3 protein ElisaKit, MACH-beta-1/2/3/4 protein ElisaKit


Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
CASP8 / Caspase 8
Reactivity
Human
Manual
Intended Sample Types
Cell Culture Supernatants, Serum
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
31.2 - 2000 pg/ml
Sensitivity
10 pg/ml
Precision
Intra-Assay: CV%<5.3% Inter-Assay: CV%<8.2%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Coated 96-well Strip Plate
  • Adhesive Plate Sealers
  • Sample Diluent
  • TMB-Substrate
  • Stop Solution
  • Standard (Lyophilized)
  • Biotinylated Detection Antibody Diluent
  • ABC Complex (100x)
  • ABC Complex Diluent
  • Biotinylated Detection Antibody (100x)
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.

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Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 8/15/2018

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