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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Human CASP10 / Caspase 10 ELISA Kit (Cell-Based ELISA) - LS-F1852 Best Seller
Catalog
Size
Price
LS-F1852-1
1 plate
$490

Popular CASP10 / Caspase 10 Elisa Kits

Reactivity: Mouse
Range: 19.531-1250 pg/ml
Reactivity: Mouse
Range: 7.813-500 pg/ml
Reactivity: Mouse
Range: 19.531-1250 pg/ml

Product Description

LS-F1852 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human CASP10 / Caspase 10 in samples of Adherent Cell Cultures. It is based upon a Cell-Based assay principle.

Specifications

Type
Cell-Based ELISA (enzyme-linked immunosorbent assay) kit
Target
CASP10 / Caspase 10
Synonyms
CASP10, ALPS2, Caspase-10, Caspase 10, CASP-10, FADD-like ICE2, FLICE2, MCH4, ICE-like apoptotic protease 4, Apoptotic protease Mch-4
Reactivity
Human
Specificity
Caspase 10
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Qualitative
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • 96-Well Cell Culture Clear-Bottom Microplate
  • 10x TBS
  • Quenching Buffer
  • Blocking Buffer
  • 10x Wash Buffer
  • 100x Anti-Caspase 10 Antibody (Rabbit Polyclonal)
  • 100x Anti-GAPDH Antibody (Mouse Monoclonal)
  • HRP-Conjugated Anti-Rabbit IgG Antibody
  • HRP-Conjugated Anti-Mouse IgG Antibody
  • Primary Antibody Diluent
  • Ready-to-Use Substrate
  • Stop Solution
  • Crystal Violet Solution
  • SDS Solution
  • Adhesive Plate Seals
Protocol
                Restrictions
                For research use only.
                About CASP10 / Caspase 10
                CASP10 / Caspase 10 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Q92851 NM_001230 NP_001221.2


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                Cell-Based ELISA Platform Overview
                Cell-Based ELISA Platform Overview

                Cell-Based ELISA Platform Overview
                Cell-Based ELISA Platform Overview

                Cell-Based ELISA Platform Overview
                Cell-Based ELISA Platform Overview

                Cell-Based ELISA Platform Overview
                Cell-Based ELISA Platform Overview

                Requested From: United States
                Date Requested: 10/17/2018

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