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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Human CAMK2A / CaMKII Alpha ELISA Kit (Sandwich ELISA) - LS-F36768
LS-F36768 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CAMK2A / CaMKII Alpha in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CAMK2A / CaMKII Alpha as low as 0.094 nanograms per millilter.
Catalog
Size
Price
LS-F36768-1
1 plate
$790

Popular CAMK2A / CaMKII Alpha Elisa Kits

Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Rat
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Lysates, Tissue Homogenates
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Tissue Homogenates
Type: Custom ELISA
Format: 96-Well Strip Plate
Reactivity: Mouse
Detection: Colorimetric - 450nm (TMB)
Type: Custom ELISA
Format: 96-Well Strip Plate
Reactivity: Rat
Detection: Colorimetric - 450nm (TMB)
Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
Reactivity: Human
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

Product Description

LS-F36768 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CAMK2A / CaMKII Alpha in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CAMK2A / CaMKII Alpha as low as 0.094 nanograms per millilter.
About CAMK2A / CaMKII Alpha
CAMK2A / CaMKII Alpha belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. Q9UQM7 NM_015981 NP_057065.2

CAMK2A ElisaKit, AlphaCaMKII ElisaKit, CaM-kinase II alpha chain ElisaKit, CaMK-II subunit alpha ElisaKit, CAMKA ElisaKit, CaM kinase II alpha ElisaKit, CaMK II alpha ElisaKit, CaMK-II alpha subunit ElisaKit, CaMKIINalpha ElisaKit, CaM kinase II alpha subunit ElisaKit, CaM kinase II subunit alpha ElisaKit, CaMKII alpha ElisaKit, CaMKIIalpha ElisaKit, KIAA0968 ElisaKit


Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
CAMK2A / CaMKII Alpha
Reactivity
Human
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.156 - 10 ng/ml
Sensitivity
0.094
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
Background
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CAMK2 a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CAMK2a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP - Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CAMK2a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CAMK2a can be calculated.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.

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Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 8/15/2018

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