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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Human Basigin / Emmprin / CD147 ELISA Kit (Sandwich ELISA) - LS-F22977
Catalog
Size
Price
LS-F22977-1
1 plate
$590
Available Discounts
5% off 5+ units

Product Description

LS-F22977 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Basigin / Emmprin / CD147 in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of Basigin / Emmprin / CD147 as low as 9.375 picograms per milliliter.

Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
Basigin / Emmprin / CD147
Reactivity
Human
Specificity
This kit recognizes natural and recombinant Human EMMPRIN/CD147.
Manual
Intended Sample Types
Plasma, Serum
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
15.625 - 1000 pg/ml
Sensitivity
9.375 pg/ml
Assay Time
3.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<10%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • User Manual
  • 96-well Microplate
  • Detection Reagents
  • Wash Buffer
  • Substrate
  • Stop Solution
  • Adhesive Plate Sealers
Protocol
  1. Prepare all reagents, samples and standards.
  2. Add 100 µl standard or sample to each well and incubate for 90 minutes at 37°C.
  3. Aspirate and add 100 µl prepared Detection Antibody and incubate of 1 hour at 37°C.
  4. Aspirate and wash 3 times.
  5. Add 100 µl HRP Conjugate and incubate for 30 minutes at 37°C.
  6. Aspirate and wash 5 times.
  7. Add 90 µl Substrate Solution and incubate for 15-20 minutes at 37°C.
  8. Add 50 µl Stop Solution.
  9. Read immediately at 450nm.
Restrictions
For research use only.
About Basigin / Emmprin / CD147
P35613 NM_001728 NP_001719.2

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Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 12/14/2018
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