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Human ATF3 ELISA Kit (Sandwich ELISA) - LS-F36735

Catalog Size Price
LS-F36735-1 1 plate Unavailable

Most Popular ATF3 Elisa Kits

Mouse/Human/Rat ATF3 ELISA Kit (DNA-Binding ELISA) - LS-F740
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Mouse/Human/Rat ATF3 ELISA Kit (Cell-Based ELISA) - LS-F1809
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Human ATF3 ELISA Kit (Sandwich ELISA) - LS-F9891
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100% Guaranteed
LS-F36735
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
ATF3
Human
LS-F36735 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human ATF3 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of ATF3 as low as 0.094 nanograms per millilter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
0.156 - 10 ng/ml
0.094
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ATF-3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- ATF-3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ATF-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ATF-3 can be calculated.
Short term: 4°C; Long term: see manual.
For research use only.

About ATF3

P18847 NM_001674 NP_004015.4

ATF3 ElisaKit

Activating transcription factor 3 (ATF3)is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. It encodes a protein with a calculated molecular mass of 22 kD. ATF3 represses rather than activates transcription from promoters with ATF binding elements.

 

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: 
Date Requested: 5/25/2018

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