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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Human APP / Beta Amyloid Precursor ELISA Kit (Sandwich ELISA) - LS-F3319
Catalog
Size
Price
LS-F3319-1
1 plate
$640
Available Discounts
10% off 2-4 units
20% off 5+ units

Popular APP / Beta Amyloid Precursor Elisa Kits


Product Description

LS-F3319 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human APP / Beta Amyloid Precursor in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of APP / Beta Amyloid Precursor as low as 0.137 nanograms per millilter.

Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
APP / Beta Amyloid Precursor
Synonyms
APP, ABPP, A4, ABETA, AD1, Alzheimer disease, Amyloid beta, Beta-amyloid peptide, APPI, Beta amyloid, CVAP, AAA, PN2, PreA4, Amyloid beta A4 protein, Peptidase nexin-II, CTFgamma, PN-II, Protease nexin-II
Reactivity
Human
Manual
Intended Sample Types
Cell Culture Supernatants, Plasma, Serum
Sample Dilution
2 fold
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.137 - 33.33 ng/ml
Sensitivity
0.137 ng/ml
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Coated 96-well Strip Plate
  • Wash Buffer Concentrate (20X)
  • Standard (Lyophilized)
  • Biotinylated Detection Antibody
  • HRP-Streptavidin Conjugate (1100x)
  • TMB One-Step Substrate Reagent
  • Stop Solution
  • Assay Diluent (5x)
  • Adhesive Plate Sealers
Protocol
                        Restrictions
                        For research use only.
                        About APP / Beta Amyloid Precursor
                        Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. P05067 NM_000484 NP_000475.1


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                        Sandwich ELISA Platform Overview
                        Sandwich ELISA Platform Overview

                        Sandwich ELISA Platform Overview
                        Sandwich ELISA Platform Overview

                        Sandwich ELISA Platform Overview
                        Sandwich ELISA Platform Overview

                        Sandwich ELISA Platform Overview
                        Sandwich ELISA Platform Overview

                        Requested From: United States
                        Date Requested: 10/18/2018

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