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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Human AKAP14 ELISA Kit (Cell-Based ELISA) - LS-F3246 Best Seller
Catalog
Size
Price
LS-F3246-1
1 plate
$490

Product Description

LS-F3246 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human AKAP14 in samples of Adherent Cell Cultures. It is based upon a Cell-Based assay principle.

Specifications

Type
Cell-Based ELISA (enzyme-linked immunosorbent assay) kit
Target
AKAP14
Reactivity
Human
Specificity
AKAP14
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Qualitative
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • User Manual
  • 96-well Cell Culture Microplate
  • Blocking Buffer
  • Detection Antibodies
  • HRP-Conjugate
  • Substrate
  • Stop Solution
  • Crystal Violet Solution
  • SDS Solution
  • Adhesive Plate Sealers
Protocol
  1. Seed cells into wells and incubate overnight at 37°C, 5% CO2.
  2. Apply desired treatment conditions.
  3. Add 100 µl of Fixing Solution and incubate 20 minutes at room temperature.
  4. Add 100 µl of Quenching Buffer and incubate 20 minutes at room temperature.
  5. Add 200 µl of Blocking Buffer and incubate for 1 hour at room temperature.
  6. Add 50 µl of 1x primary antibodies and incubate overnight at 4°C.
  7. Add 50 µl of HRP-conjugated secondary antibodies and incubate for 1.5 hours at room temperature.
  8. Add 50 µl of Ready-to-Use Substrate and incubate for 30 minutes at room temperature.
  9. Add 50 µl of Stop Solution and read OD at 450 nm.
Restrictions
For research use only.
About AKAP14
Q86UN6 AA883165 Q86UN6

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Cell-Based ELISA Platform Overview
Cell-Based ELISA Platform Overview

Cell-Based ELISA Platform Overview
Cell-Based ELISA Platform Overview

Cell-Based ELISA Platform Overview
Cell-Based ELISA Platform Overview

Cell-Based ELISA Platform Overview
Cell-Based ELISA Platform Overview

Requested From: United States
Date Requested: 12/15/2018
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