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Human Adiponectin ELISA Kit (Sandwich ELISA) - LS-F10042

Ordering

Size Price
1 plate $640
Discounts 5+ units 10%
Inquire for larger quantities
LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Adiponectin Elisa Kits

Mouse Adiponectin ELISA Kit (CLIA) - LS-F26023
Quantitative Sandwich CLIA ELISA
96-Well Strip Plate
Mouse
5.49-4000 pg/ml
Chemiluminescent
Cell Culture Supernatants, Cell Lysates, Serum, Tissue Homogenates, Plasma
 Image
Human Adiponectin ELISA Kit (CLIA) - LS-F26025
Quantitative Sandwich CLIA ELISA
96-Well Strip Plate
Human
13.72-10000 pg/ml
Chemiluminescent
Cell Culture Supernatants, Serum, Tissue Homogenates, Plasma
 Image
Bovine Adiponectin ELISA Kit (Sandwich ELISA) - LS-F6080
Sandwich ELISA
96-Well Strip Plate
Bovine
0.781-50 ng/ml
Colorimetric - 450nm
Serum, Plasma
 Image

100% Guaranteed 100% Guaranteed
LS-F10042
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Adiponectin
Human
This assay has high sensitivity and excellent specificity for detection of human ADP. No significant cross-reactivity or interference between human ADP and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human ADP and all the analogs, therefore, cross reaction may still exist.
LS-F10042 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Adiponectin in samples of Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates and Urine. It is based upon a Sandwich assay principle and can be used to detect levels of Adiponectin as low as 1.102 nanograms per millilter.
All LSBio Custom kits have been predesigned and all necessary components, including antibodies and standards have been identified. Upon receiving a custom kit order, the kit will be assembled and quality control tested before being shipped out. Kit assembly and testing typically takes 4 to 6 weeks. In most cases the final kit is based on the Sandwich assay principle, with a few being Competitive EIA based. Specifications such as Range, Sensitivity, and Precision are defined upon completion. In the event that the custom kit cannot be successfully developed with 6 weeks of the order date, the customer will be notified and the order canceled at no cost.
Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates, Urine
50 - 100 µl
96-Well Microplate
Quantitative
1.56 - 100 ng/ml
1.102 ng/ml
Intra-assay: CV%<8% Inter-assay: CV%<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping. ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Assay plate (12 x 8 coated Microwells)
  • Standard (Lyophilized)
  • Biotin-antibody (100 x concentrate)
  • HRP-avidin (100 x concentrate)
  • Biotin-antibody Diluent
  • HRP-avidin Diluent
  • Sample Diluent
  • Wash Buffer (25 x concentrate)
  • TMB Substrate
  • Stop Solution
  • Adhesive Strip (For 96 wells)
    This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADP bound in the initial step. The color development is stopped and the intensity of the color is measured.
2°C to 8°C
For research use only.

About Adiponectin

Q15848 NM_004797 NP_004788.1

ADIPOQ Elisa Kit, Adiponectin Elisa Kit, ADPN Elisa Kit, ACDC Elisa Kit, ADIPQTL1 Elisa Kit, APM-1 Elisa Kit, APM1 Elisa Kit, ACRP30 Elisa Kit, Gelatin-binding protein 28 Elisa Kit, GBP28 Elisa Kit, Gelatin-binding protein Elisa Kit

Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects.

Requested From: United States
Date Requested: 12/8/2016

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