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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Human A2M / Alpha-2-Macroglobulin ELISA Kit (Sandwich ELISA) - LS-F2835
LS-F2835 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human A2M / Alpha-2-Macroglobulin in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of A2M / Alpha-2-Macroglobulin as low as 20 picograms per milliliter.
Catalog
Size
Price
LS-F2835-1
1 plate
$530

Popular A2M / Alpha-2-Macroglobulin Elisa Kits

Type: Competitive EIA
Format: 96-Well Strip Plate
Reactivity: Mouse
Range: 3.7-300 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Urine, Plasma
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Mouse
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 0.78-50 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Urine, Plasma
Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
Reactivity: Human
Range: 12.29-1200 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Serum, Plasma

Product Description

LS-F2835 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human A2M / Alpha-2-Macroglobulin in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of A2M / Alpha-2-Macroglobulin as low as 20 picograms per milliliter.
About A2M / Alpha-2-Macroglobulin
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). P01023 NM_000014 NP_000005.2

A2M ElisaKit, CPAMD5 ElisaKit, A2MD ElisaKit, FWP007 ElisaKit, Alpha-2-M ElisaKit, Alpha-2-macroglobulin ElisaKit, S863-7 ElisaKit


Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
A2M / Alpha-2-Macroglobulin
Reactivity
Human
Specificity
This kit is for the detection of natural human A2M. No significant cross-reactivity or interference between A2M and analogs was observed.
Manual
Intended Sample Types
Cell Culture Supernatants, Plasma, Serum
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
625 - 40000 pg/ml
Sensitivity
20 pg/ml
Precision
Intra-Assay: CV%<6.9% Inter-Assay: CV%<7.9%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Coated 96-well Strip Plate
  • Standard (Lyophilized)
  • Biotinylated Detection Antibody (100x)
  • ABC Complex (100x)
  • Sample Diluent
  • Detection Antibody Diluent
  • ABC Complex Diluent
  • TMB Substrate
  • Stop Solution
  • Adhesive Plate Sealers
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.

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Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 8/19/2018

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