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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Chicken IL8 / Interleukin 8 ELISA Kit (Sandwich ELISA) - LS-F36638
Catalog
Size
Price
LS-F36638-1
1 plate
$690

Popular IL8 / Interleukin 8 Elisa Kits

Reactivity: Bovine
Range: 31.2-2000 pg/ml
Reactivity: Chicken
Range: 15.6-1000 pg/ml
Reactivity: Human
Range: 31.25-2000 pg/ml
Reactivity: Feline, Cat
Range: 25-6000 pg/ml
Reactivity: Rabbit
Range: 15.63-1000 pg/ml

Product Description

LS-F36638 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Chicken IL8 / Interleukin 8 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of IL8 / Interleukin 8 as low as 9.375 picograms per milliliter.

Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
IL8 / Interleukin 8
Synonyms
CXCL8, 3-10C, AMCF-I, B-ENAP, C-X-C motif chemokine 8, GCP-1, IL-8, IL8, Interleukin-8, K60, LECT, GCP1, LUCT, NAF, NAP1, Neutrophil-activating factor, Interleukin 8, Protein 3-10C, MDNCF, TSG-1, Emoctakin, LYNAP, MONAP, NAP-1, SCYB8, T-cell chemotactic factor
Reactivity
Chicken
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
15.625 - 1000 pg/ml
Sensitivity
9.375 pg/ml
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
Store at 4°C. Stable for 6 months.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
Background
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-8 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-8 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-8 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-8 can be calculated.
Restrictions
For research use only.
About IL8 / Interleukin 8
Interleukin 8 (IL8), a member of the CXC chemokine family. This chemokine, is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. P10145 NM_000584 NP_000575.1


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Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 10/17/2018

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